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model p 87 flaming brown micropipette puller  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company model p 87 flaming brown micropipette puller
    Model P 87 Flaming Brown Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 2430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micropipette+puller+p-87/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pmc12381851-79-26-23
    Average 96 stars, based on 2430 article reviews
    model p 87 flaming brown micropipette puller - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Microscopy:

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda
    Article Snippet: The tissue was then dipped into ice‐cold Ringer's solution and washed in Ringer's solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P‐87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F‐8P, Science Products, Hofheim, Germany) and with their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda.
    Article Snippet: The tissue was then dipped into ice-cold Ringer’s solution and washed in Ringer’s solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P-87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F-8P, Science Products, Hofheim, Germany) andwith their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    other:

    Article Title: Membrane Interfacial Potential Governs Surface Condensation and Fibrillation of α-Synuclein in Neurons
    Article Snippet: The micropipettes were pulled from 10 μm diameter glass capillaries using a Sutter Instruments P-87 puller and a Narishige Microscope MF-900.

    Article Title: Inhibition the ubiquitination of ENaC and Na,K-ATPase with erythropoietin promotes alveolar fluid clearance in sepsis-induced acute respiratory distress syndrome.
    Article Snippet: Pipettes were pulled from the capillary glass electrode with a P-87 micropipette puller (Sutter Instrument Company), and the resistance varied from 3 to 5 MΩ when filled with this micropipette internal solution.

    Imaging:

    Article Title: SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.
    Article Snippet: Rpe1-Halo_CenpA cells (gift from A. McAinsh, Warwick Medical School27) were cultured in DMEM/F12 (Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, Thermo Fisher Scientific, 11320082) supplemented with 10% qualified and heat-inactivated fetal bovine serum (FBS) (10438-026, Gibco), 5% penicillin/streptomycin and 300ug/ml Geneticin (G418 sulfate, Gibo, 11811-031), and maintained at 37oC and 5% CO2. .. Glass capillaries were pulled with micropipette puller (P-87, Sutter Instruments), bent and polished using a microforge (Narishige International) according to the same specifications, parameters, and geometries described earlier.36 These parameters allowed for the needle to approach cells orthogonal to the imaging plane and to conduct manipulations without rupturing Current Biology 35, 1–11.e1–e4, April 21, 2025 e2 Article the cell.36 Prior to imaging, microneedles were coated with BSA-Alexa-488 (Invitrogen, A13100) and BSA-Alexa-555 (Invitrogen, A34786) by soaking them in coating solution for 60 s. Coating solution was obtained by dissolving BSA-Alexa dye and Sodium Azide (Nacalai Tesque) in 0.1 M phosphate-buffered saline (PBS) at a final concentration of 0.02% and 3 mM, respectively.50 This coating solution allows needles to be visualized via fluorescence imaging, aiding in positioning of the needle along a single k-fiber, near to, but not touching, the kinetochore at a z-height appropriate for pulling on k-fibers without overly deforming the membrane. .. For manipulation experiments, the Tokai Hit incubation chamber lid was removed to allow for needle access.

    Saline:

    Article Title: SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.
    Article Snippet: Rpe1-Halo_CenpA cells (gift from A. McAinsh, Warwick Medical School27) were cultured in DMEM/F12 (Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, Thermo Fisher Scientific, 11320082) supplemented with 10% qualified and heat-inactivated fetal bovine serum (FBS) (10438-026, Gibco), 5% penicillin/streptomycin and 300ug/ml Geneticin (G418 sulfate, Gibo, 11811-031), and maintained at 37oC and 5% CO2. .. Glass capillaries were pulled with micropipette puller (P-87, Sutter Instruments), bent and polished using a microforge (Narishige International) according to the same specifications, parameters, and geometries described earlier.36 These parameters allowed for the needle to approach cells orthogonal to the imaging plane and to conduct manipulations without rupturing Current Biology 35, 1–11.e1–e4, April 21, 2025 e2 Article the cell.36 Prior to imaging, microneedles were coated with BSA-Alexa-488 (Invitrogen, A13100) and BSA-Alexa-555 (Invitrogen, A34786) by soaking them in coating solution for 60 s. Coating solution was obtained by dissolving BSA-Alexa dye and Sodium Azide (Nacalai Tesque) in 0.1 M phosphate-buffered saline (PBS) at a final concentration of 0.02% and 3 mM, respectively.50 This coating solution allows needles to be visualized via fluorescence imaging, aiding in positioning of the needle along a single k-fiber, near to, but not touching, the kinetochore at a z-height appropriate for pulling on k-fibers without overly deforming the membrane. .. For manipulation experiments, the Tokai Hit incubation chamber lid was removed to allow for needle access.

    Concentration Assay:

    Article Title: SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.
    Article Snippet: Rpe1-Halo_CenpA cells (gift from A. McAinsh, Warwick Medical School27) were cultured in DMEM/F12 (Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, Thermo Fisher Scientific, 11320082) supplemented with 10% qualified and heat-inactivated fetal bovine serum (FBS) (10438-026, Gibco), 5% penicillin/streptomycin and 300ug/ml Geneticin (G418 sulfate, Gibo, 11811-031), and maintained at 37oC and 5% CO2. .. Glass capillaries were pulled with micropipette puller (P-87, Sutter Instruments), bent and polished using a microforge (Narishige International) according to the same specifications, parameters, and geometries described earlier.36 These parameters allowed for the needle to approach cells orthogonal to the imaging plane and to conduct manipulations without rupturing Current Biology 35, 1–11.e1–e4, April 21, 2025 e2 Article the cell.36 Prior to imaging, microneedles were coated with BSA-Alexa-488 (Invitrogen, A13100) and BSA-Alexa-555 (Invitrogen, A34786) by soaking them in coating solution for 60 s. Coating solution was obtained by dissolving BSA-Alexa dye and Sodium Azide (Nacalai Tesque) in 0.1 M phosphate-buffered saline (PBS) at a final concentration of 0.02% and 3 mM, respectively.50 This coating solution allows needles to be visualized via fluorescence imaging, aiding in positioning of the needle along a single k-fiber, near to, but not touching, the kinetochore at a z-height appropriate for pulling on k-fibers without overly deforming the membrane. .. For manipulation experiments, the Tokai Hit incubation chamber lid was removed to allow for needle access.

    Fluorescence:

    Article Title: SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.
    Article Snippet: Rpe1-Halo_CenpA cells (gift from A. McAinsh, Warwick Medical School27) were cultured in DMEM/F12 (Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, Thermo Fisher Scientific, 11320082) supplemented with 10% qualified and heat-inactivated fetal bovine serum (FBS) (10438-026, Gibco), 5% penicillin/streptomycin and 300ug/ml Geneticin (G418 sulfate, Gibo, 11811-031), and maintained at 37oC and 5% CO2. .. Glass capillaries were pulled with micropipette puller (P-87, Sutter Instruments), bent and polished using a microforge (Narishige International) according to the same specifications, parameters, and geometries described earlier.36 These parameters allowed for the needle to approach cells orthogonal to the imaging plane and to conduct manipulations without rupturing Current Biology 35, 1–11.e1–e4, April 21, 2025 e2 Article the cell.36 Prior to imaging, microneedles were coated with BSA-Alexa-488 (Invitrogen, A13100) and BSA-Alexa-555 (Invitrogen, A34786) by soaking them in coating solution for 60 s. Coating solution was obtained by dissolving BSA-Alexa dye and Sodium Azide (Nacalai Tesque) in 0.1 M phosphate-buffered saline (PBS) at a final concentration of 0.02% and 3 mM, respectively.50 This coating solution allows needles to be visualized via fluorescence imaging, aiding in positioning of the needle along a single k-fiber, near to, but not touching, the kinetochore at a z-height appropriate for pulling on k-fibers without overly deforming the membrane. .. For manipulation experiments, the Tokai Hit incubation chamber lid was removed to allow for needle access.

    Membrane:

    Article Title: SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.
    Article Snippet: Rpe1-Halo_CenpA cells (gift from A. McAinsh, Warwick Medical School27) were cultured in DMEM/F12 (Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, Thermo Fisher Scientific, 11320082) supplemented with 10% qualified and heat-inactivated fetal bovine serum (FBS) (10438-026, Gibco), 5% penicillin/streptomycin and 300ug/ml Geneticin (G418 sulfate, Gibo, 11811-031), and maintained at 37oC and 5% CO2. .. Glass capillaries were pulled with micropipette puller (P-87, Sutter Instruments), bent and polished using a microforge (Narishige International) according to the same specifications, parameters, and geometries described earlier.36 These parameters allowed for the needle to approach cells orthogonal to the imaging plane and to conduct manipulations without rupturing Current Biology 35, 1–11.e1–e4, April 21, 2025 e2 Article the cell.36 Prior to imaging, microneedles were coated with BSA-Alexa-488 (Invitrogen, A13100) and BSA-Alexa-555 (Invitrogen, A34786) by soaking them in coating solution for 60 s. Coating solution was obtained by dissolving BSA-Alexa dye and Sodium Azide (Nacalai Tesque) in 0.1 M phosphate-buffered saline (PBS) at a final concentration of 0.02% and 3 mM, respectively.50 This coating solution allows needles to be visualized via fluorescence imaging, aiding in positioning of the needle along a single k-fiber, near to, but not touching, the kinetochore at a z-height appropriate for pulling on k-fibers without overly deforming the membrane. .. For manipulation experiments, the Tokai Hit incubation chamber lid was removed to allow for needle access.



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